Calcium channel blocker and renal mitochondrial function in warm renal ischemia

OBJECTIVE: Ions, particularly calcium ions, play an important role in ischemia-reperfusion cell injury. In this study, we investigated the action of verapamil on the mitochondrial function of kidneys submitted to ischemia without blood reperfusion in order to study isolated early and late ischemic effects. MATERIALS AND METHODS: 44 rats were submitted to bilateral warm renal ischemia for 30 minutes. The kidneys were then immediately reperfused with saline or Euro-Collins (EC) solution, with and without previous administration of 0.35 mg/kg of verapamil. Mitochondrial function was assessed at the end of renal perfusion and after 24 hours of cold preservation. RESULTS: In kidneys perfused with saline, verapamil allowed a significant early preservation of state III mitochondrial respiration, a result that was no longer evident after 24 hours. In kidneys perfused with EC solution, verapamil did not change state III for either early or late evaluations. Comparison of the groups showed that the results obtained for kidneys perfused with EC were always superior to those obtained for the saline group, except for the initial analysis of kidneys treated with saline and verapamil, which showed results similar to those obtained with EC perfusion alone. CONCLUSION: Administration of verapamil before warm ischemia provides partial and short-lasting functional protection of the mitochondrial function in kidneys perfused with sodium rich saline. With Euro-Collins solution, verapamil did not show any additional beneficial effect. This fact permits us to conclude that protective action is effective only under conditions that facilitate increased sodium uptake and/or potassium loss.

Análise comparativa da reserva de glicogênio do miocárdio isquêmico de coelhos submetidos a cardioplegia hipotérmica ou normotérmica / Comparative analysis of glycogen storage of ischemic myocardium of rabbits sujected to hypothermia or normothermic cardioplegia

Neste trabalho, a comparaçao da reserva de glicogênio é utilizada para entender as modificaçoes na sensibilidade mitocondrial ao íon cálcio. Basicamente porque alteraçoes mitocondriais que envolvam perda da atividade de fosforilaçao oxidativa provocam rápida utilizaçao dos estoques de glicogênio. Nossos resultados sugerem que as alteraçoes mitocondriais fazem parte do mecanismo de lesao isquêmica.